Consejo
Superior de Investigaciones Ceintíficas
Instituto de Agroquímica
y Tecnología de Alimentos
Departamento de Biotecnología de los Alimentos
Polígono de la Coma s/n. Apartado de correos 73
46100 Burjasot (Valencia)
ESPAÑA
Telefone: + 34 96 3900022 - ext.
2306
Fax: + 34 96 3636301
Representante: Amparo Querol Simón
Email: aquerol@iata.csic.es
COMPETÊNCIAS
Enologia - Aditivos e Práticas
Enológicas
Enologia - Análise de Contaminantes
Enologia - Compostos de Aroma
Enologia - Leveduras
Enologia - Técnicas de biologia molecular aplicada a microorganismos
EQUIPA
Agustín Aranda Fernández
Amparo Querol Simón
Eladio Barrio Esparducer
Emilia Matallana Redondo
Marcellí del Olmo Muñoz
Maria José Peris Torán
Maria Teresa Fernández Espinar García
PUBLICAÇÕES REPRESENTATIVAS
DE LLANOS, R,. FERNÁNDEZ-ESPINAR,
M.T., QUEROL, A. (2004): Identification of species of the genus
Candida by RFLP analysis of the 5.8S rRNA gene and the two ribosomal
internal transcribed spacers. Antonie van Leeuwenhoek. 85:175-185.
DE LLANOS, R.; FERNÁNDEZ-ESPINAR,
M.T.; PLANES, A.N.; QUEROL, A. (2004): Molecular characterization
of clinical and non-clinical isolates of Saccharomyces cerevisiae.
Systematic Appl. Microbiol. (En prensa)
ESTEVE-ZARZOSO, B., FERNÁNDEZ-ESPINAR,
M.T., QUEROL, A.(2004): Authentication and identification of Saccharomyces
cerevisiae "flor" yeast races involved in sherry aging.
Antonie van Leeuwenhoek. 85:151-158.
MORRISSEY, W.F., QUEROL, A.; DOBSON, A. (2004):
The role of indigenous yeasts in traditional Irish cider fermentations.
J Appl Microbiol (Aceptado).
QUEROL, A., FERNÁNDEZ-ESPINAR, M.T.;
BARRIO, E. (2004): Genetic variability of yeast in wine fermentation.
In: Mycology, Vol. 21: Fungal Biotechnology in Agricultural,
Food, an Environmental Applications. Marcer Dekker, Inc. (Dilip
D. Arora, Ed.). Nerw York (USA). ISBN: 0-8247-4770-4. 257-267.
ZUZUARREGUI, A; DEL OLMO, M. (2004): Analyses
of stress resistance under laboratory conditions constitute a suitable
criterion for wine yeasts selection. Antonie van Leeuwnhoek.
85(4):271-280.
ZUZUAREGUI, A; DEL OLMO, M. (2004): Expression
of stress response genes in wine strains with different fermentative
behavior. Fems Yeast Res. 4(7): 699-710.
ALLER-ARRANZ, E., RANDEZ-GIL, F., BARRIO,
E., PRIETO, JA. (2003): A DNA region of Torulaspora delbrueckii
containing the HIS3 gene sequence, gene order and evolution. Yeast.
20(16): 1359-1368.
ARANDA, A.; DEL OLMO, M. (2003): Response
to acetaldehyde stress in the yeast Saccharomyces cerevisiae involves
a strain dependent regulation of several ALD genes and is mediated
by the general stress response pathway. Yeast 20:747-759.
CARRASCO, P., PÉREZ-ORTÍN,
J.E., DEL OLMO, M. (2003): Arginase activity is a useful marker
of nitrogen limitation during alcoholic fermentations. Systematic
Appl. Microbiol. 26: 471-479.
DIAS, L., DIAS, S., SANCHO, T., STENDER,
H., QUEROL, A., MALFEITO-FERREIRA, M.; LOUREIRO, V. (2003): Identification
of yeasts isolated from wine-related environments and capable of
producing 4-ethylphenol. Food Microbiology. 20:567-574.
ESTEVE-ZARZOSO, B., ZORMAN, T., BELLOCH,
C., AND QUEROL, A. (2003): Molecular characterisation of the species
of the genus Zygosacccharomyces. System. Appl. Microbiol. 26:404-411.
FERNÁNDEZ-ESPINAR, T, BARRIO, E.,
RAMÓN D.; QUEROL, A. (2003): Analysis of the genetic variability
in the species of Saccharomyces sensu stricto complex. Yeast.
20, 1213-1226.
MARTÍNEZ-CULEBRAS, P.V., QUEROL, A.,
SUÁREZ-FERNÁNDEZ, M.B., GARCÍA, M.D., BARRIO,
E. (2003): Phylogenetic relationships among Colletotrichum pathogens
of strawberry and design of PCR primers for their identification.
J. Phytopathol. 151(3):135-143.
QUEROL, A., FERNÁNDEZ-ESPINAR, M.T.,
DEL OLMO, M., BARRIO, E. (2003): Molecular evolution in yeast of
biotechnological interest. Int. Microbiol. 6, 201-205.
QUEROL, A., FERNÁNDEZ-ESPINAR, M.T.,
DEL OLMO, M., BARRIO, E. (2003): Adaptive
evolution of wine yeast. Int. J. Food. Microbiol. 86: 3-10.
SÁEZ, A.G., TATARENKOV, A., BARRIO,
E., BECERRA, N.H., AYALA, F.J. (2003): Patterns of DNA sequence
polymorphism at Sod vicinities in Drosophila melanogaster: Unraveling
the footprint of a recent selective sweep. Proc. Natl. Acad.
Sci. USA. 100 (4): 1793-1798.
ALÉN, C., KENT, N.A., JONES, H.S.,
O'SULLIVAN, J., ARANDA, A. PROUDFOOT, N.J. (2002): A Role for Chromatin
Remodeling in Transcriptional Termination by RNA Polymerase II.
Mol. Cell. 10: 1441-1452.
ARANDA, A., QUEROL, A.; OLMO, M. DEL. (2002):
Correlation between acetaldehyde and ethanol resistance and expression
of HSP genes in yeast strains isolated during the biological aging
of sherry wines. Arch Microbiol. 177: 304-312.
BELLOCH, C., FERNÁNDEZ-ESPINAR, T.
QUEROL, A., GARCÍA, M.D. BARRIO, E. (2002): An analysis of
inter-and intraspecific genetic variabilities in the K. marxianus
group of yeast species for the reconsideration of the K. lactis
taxon. Yeast. 19: 257-268.
CAMPBELL, S.G., DEL OLMO, M., BEGLAN, P.,
BOND, U. (2002): A sequence element downstream of the yeast HTB1
gene contributes to mRNA 3' processing and cell cycle regulation.
Molecular and Cellular Biology. 22:8415-8425.
FARES, M.A., BARRIO, E., SABATER-MUÑOZ,
B., MOYA, A. (2002): The evolution of the heat-shock protein GroEL
from Buchnera, the primary endosymbiont of aphids, is governed by
positive selection. Molecular Biology and Evolution 19:1162-1170.
FARES, M.A., ELENA, S.F., ORTIZ, J., MOYA,
A., BARRIO, E. (2002): A sliding window-based method to detect selective
constraints in protein coding genes: highlighting positively selected
regions in RNA viruses. Journal of Molecular Evolution. 55:509-521.
FARES, M.A., RUIZ-GONZÁLEZ, M.X.,
MOYA, A., ELENA, S.F., BARRIO, E. (2002): Endosymbiotic bacteria:
GroEL buffers against deleterious mutations. Nature 417:398.
GONZÁLEZ-ÁLVAREZ, M., ALZUET,
G., BORRÁS, J., MACÍAS, B., DEL OLMO, M., LIU-GONZÁLEZ,
M.; SANZ, F. (2002): Nuclease activity of (Cu(sulfathiazolato)2(benzimidazole)2)2MeOH.
Synthesis, properties and crystal structure. Journal of Inorganic
Biochemistry. 89: 29-35.
LOPES, C.A., BROOCK, M. VAN., QUEROL, A.,
CABALLERO, A.C. (2002): Saccharomyces cerevisiae wine yeast populations
in a cold region in Argentinean Patagonia. A study at different
fermentation scales. J Applied Microbiology. 93:608-615.
LÓPEZ, V., FERNÁNDEZ-ESPINAR,
M.T., BARRIO, E., RAMÓN, D. QUEROL, A. (2002): A new PCR-based
method for monitoring inoculated wine fermentations. Int J Food
Microbiology. 81: 63-71.
MARTÍNEZ-CULEBRAS, P.V., SUÁREZ-FERNÁNDEZ,
M.B., BARRIO, E., GARCÍA, M.D., QUEROL, A. (2002): RAPDs
analysis of Colletotrichum species derived from strawberry and specific
primers for identification of C. fragariae species. J. Phytopathol.
150:680-686.
PEDREÑO, Y., GIMENO-ALCAÑÍZ,
J.V., MATALLANA, E., ARGÜELLES, J.C. (2002): Response to oxidative
stress caused by H2O2 in saccharomyces cerevisiae mutants deficient
in trehalase genes. Arch. Microbiol
PÉREZ-ORTÍN, J.E.; QUEROL A.,
PUIG, S.; BARRIO, E. (2002): Molecular Characterization of a Chromosomal
Rearrangement Involved in the Adaptive Evolution of Wine Yeast Strains.
Genome Research. 12:1533-1539.
PÉREZ-TORRADO, R., CARRACO, P., GIMENO-ALCAÑÍZ,
J.V., PÉREZ-ORTÍN, J.E., MATALLANA, E.; DEL OLMO,
M. (2002): Study of the first hours of microvinification by the
use of osmotic stress-response genes as probes. System. Appl.
Microbiol. 25:153-161.
PÉREZ-TORRADO, R., GIMENO-ALCAÑIZ,
J.V. MATALLANA, E. (2002): Wine yeast strains engineered for glycogen
overproduction display enhanced viability under glucose deprivation
conditions. Appl. Environm. Microbiol.
ARANDA, A., PROUDFOOT, N.J. (2001): Transcriptional
termination factors for RNA polynerase II in yeast. Mol. Cell.
7:1003-1011.
CARRASCO, P., QUEROL, A., DEL OLMO, M. (2001):
Analysis of the stress resistance of commercial wine yeast strains.
Arch Microbiol. 175, 450-457.
ESTEVE-ZARZOSO, B., PERIS-TORÁN, M.J.,
GARCÍA-MAIQUEZ, E., URUBURU, F. ; QUEROL, A. (2001): Yeast
population dynamics during the fermentation and biological aging
of sherry wines. Appl. Environ. Microbiol. 67(5), 2056-2061.
ESTEVE-ZARZOSO, B., PERIS-TORÁN, M.J.,
RAMÓN, D., QUEROL, A. (2001): Molecular characterization
of Hanseniaspora species. Antonie van Leeuwenhoek. 80: 85-92.
FARES, M.A., MOYA, A., ESCARMÍS, C.,
BARANOWSKI, E., DOMINGO, E., BARRIO, E. (2001): Evidence for positive
selection in the capsid protein-coding region of the foot-and-mouth
disease virus (FMDV) subjected to experimental passage regimens.
Molecular Biology and Evolution. 18(1): 10-21.
FERNÁNDEZ-ESPINAR, M.T., LÓPEZ,
V., RAMÓN, D., BARTRA, E., QUEROL, A. (2001): Study of the
authenticity of commercial wine yeast strains by molecular techniques.
International Journal of Food Microbiology. 70:1-10.
GANGA, A., PIÑAGA, F., QUEROL, A.,
RAMÓN, D. VALLÉS, S. (2001): Cell-wall degrading enzymes
in the release of grape aroma precursors. Food Sci. Technol.
Int. 7(1):83-87.
GIMENO-ALCAÑIZ, J.V., MATALLANA, E.
(2001): Performance of industrial strains of Saccharomyces cerevisiae
during wine fermentation is affected by manipulation strategies
based on sporulation. System. Appl. Microbiol. 24, 639-644.
LÓPEZ, V., QUEROL, A., RAMÓN,
D., FERNÁNDEZ-ESPINAR, M.T. (2001): A simplified procedure
to analyse mitochondrial DNA from industrial yeasts. International
Journal of Food Microbiology. 68:75-81.
MARÍN, I., FARES, M.A., GONZÁLEZ-CANDELAS, L., BARRIO,
E., MOYA, A. (2001): Detecting changes in the functional constrints
of paralogous genes. Journal of Molecular Evolution. 52:17-28.
BELLOCH, C., QUEROL, A., GARCÍA, M.D.,
BARRIO, E. (2000): Phylogeny of the genus Kluyveromyces inferred
from the mitochondrial cytochrome-c oxidase II gene. Int. J.
of Syst. and Evolutionary Microbiology. 50: 405-416.
ESTEVE-ZARZOSO, B.; GOSTÍNCAR, A.;
BOBET, R.; URUBURU, F.; QUEROL, A. (2000): Selection and molecular
characterization of wine yeasts isolated from "El Penedes"
area (Spain). Food Microbiology. 17:553-562.
FERNÁNDEZ-ESPINAR, M.T., ESTEVE-ZARZOSO,
B., QUEROL, A. BARRIO, E. (2000): RFLP analysis of the ribosomal
internal transcribed spacer and the 5.8S rRNA gene region of the
genus Saccharomyces: a fast method for species identification and
the differentiation of flor yeasts. Anton Leeuw Int. J.G. 78:87-97.
FERNÁNDEZ-ESPINAR, T., QUEROL, A.,
RAMÓN, D. (2000): Molecular characterization of yeast strains
by mitochondrial DNA restriction analysis. ISBN 0-89603-867-X. Methods
in Biotechnology.
GREGER, I.H., ARANDA, A.; PROUDFOOT, N.J.
(2000): Balancing transcriptional interference and initiation on
the GAL7 promoter of Saccharomyces cerevisiae. Proc.Natl. Acad.
Sci. USA. 97: 8415-8420.
MARTÍNEZ-CULEBRAS, P.V., BARRIO, E.,
GARCÍA-LÒPEZ, M.D., QUEROL, A. (2000): Identification
of Collectotrichum species responsible for anthracnose of strawberry
based on the internal transcribed spacers of the ribosomal region.
FEMS Microbiology Letters. 189: 97-101.
MOYA, A., ELENA, S.F., BRACHO, A., MIRALLES,
R., BARRIO, E. (2000): The evolution of RNA viruses: a population
genetics view. Proc. Natl. Acad. Sci. USA 97(13):6967-6973.
PERAPOCH, J., PLANES, A.M., QUEROL, A., LÓPEZ,
V., MARTÍNEZ-BENDAYÁN, I., TORMO, R., FERNÁNDEZ,
F., PEGUERO, G. SALCEDO, S. (2000): Fungemia with Saccharomyces
cerevisiae in two newborns, only one of whom had been treated with
ultra-levura. Eur J Clin Microbiol Infect Dis. 19: 468-470.
PUIG, S., QUEROL, A., BARRIO, E., PÉREZ-ORTÍN,
J.E. (2000): Mitotic recombination and genetic changes in Saccharomyces
cerevisiae during wine fermentation. Applied and Environmental
Microbiology. 66(5): 2057-2061.
QUEROL, A. (2000): Modificació genètica
de llevats vínics. ACE Revista d'Enologia. 52: 16-19.
QUEROL, A., ESTEVE-ZARZOSO, B., LÓPEZ,
V., FERNÁNDEZ-ESPINAR, M.T., BARRIO, E., PERIS-TORÁN,
M.J.; RAMÓN, D. (2000): Molecular methods for the characterisation
and monitoring of wine yeasts. Inoculation Rate and nutritional
aspects keys points for food alcoholic fermentation management.
(Symposium LALLEMAND). 35-41.
VAN HELDEN, J., DEL OLMO, M., PÉREZ-ORTÍN,
J.E. (2000): Statistical analysis of yeast genomic downstream sequences
reveals putative polyadenylation signals. Nuc. Acids Res.
28: 1000-1010.
ARANDA, A., PROUDFOOT, N.J. (1999): Definition of transcriptional
pause elements in fission yeast. Mol. Cell. Biol. 19: 1595-1604.
BUDDIE, A. G., MARTÍNEZ-CULEBRAS,
P., BRIDGE, P. D., GARCÍA, M. D., QUEROL, A., CANNON, P.
F., MONTE, E. (1999): Molecular characterization of Colletotrichum
strains derived from strawberry. Mycol. Res. 103:385-394.
ESTEVE-ZARZOSO, B., BELLOCH, C., URUBURU,
F. , QUEROL, A. (1999): Identification of yeast by RFLP analysis
of the 5.8S rRNA gene and the two ribosomal internal transcribed
spacers. Int. J. Syst. Bacteriol. 49:329-337.
GANGA, M.A., PIÑAGA, F., VALLÉS,
S., RAMÓN, D., QUEROL, A. (1999): Aroma improving in microvinification
processes by the use of a recombinant wine yeast strain expressing
the Aspergillus nidulans xlnA gene. International Journal of
Food Microbiology. 47:171-178.
GASENT-TAMÍREZ, CASTREEJÓN,
F., QUEROL, A., RAMÓN, D., BENÍTEZ, T. (1999): Genomic
stability of Saccharomyces cerevisiae baker´s yeasts. System.
Appl. Microbiol. 22: 329-340.
GIMENO-ALCAÑIZ, J.V.; PÉREZ-ORTÍN,
J.E., MATALLANA, E. (1999): Differential pattern of trehalose accumulation
in wine yeast strains during the microvinification process. Biotechnol.
Lett. 21: 271-274.
IVORRA, C., PÉREZ-ORTÍN, J.E.,
DEL OLMO, M. (1999): Reverse correlation of stress-resistance ability
and stuck fermentations in wine yeasts. A molecular study. Biotechnol.
& Bioeng. 64: 678-708.
LOUREIRO, V., QUEROL, A. (1999): The prevalence
and control of spoilage yeasts in foods and beverages. Trends
in Food Science and Technology. 10, 356-365.
MANZANARES, P., RAMÓN, D., QUEROL,
A. (1999): Screening of non-Saccharomyces wine yeasts for the production
of b-D-xylosidase activity. International. J. Food Microbiol.
46, 105-112.
PUIG, S.; MATALLANA, E., PÉREZ-ORTÍN,
J.E. (1999): Stochastical mucleosome positioning in a yeast chromayin
region is not dependent on histone H1. Curr. Microbiol. 39:168-172.
PUIG, S.; PÉREZ-ORTÍN, J.E.,
MATALLANA, E. (1999): Transcriptional and structural study of a
region of two convergent overlapping yeast genes. Curr. Microbiol.
39:369-373.
TATARENKOV, A., KWIATOWSKI, J., SKARECKY, D., BARRIO, E., AYALA,
F.J. (1999): On the evolution of Ddc and Drosophila Systematics.
Journal of Molecular Evolution. 48:445-462.
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