Consejo Superior de Investigaciones Ceintíficas

Instituto de Agroquímica y Tecnología de Alimentos
Departamento de Biotecnología de los Alimentos
Polígono de la Coma s/n. Apartado de correos 73
46100 Burjasot (Valencia)
ESPAÑA

Telefone: + 34 96 3900022 - ext. 2306
Fax: + 34 96 3636301
Representante: Amparo Querol Simón
Email: aquerol@iata.csic.es


COMPETÊNCIAS

Enologia - Aditivos e Práticas Enológicas
Enologia - Análise de Contaminantes
Enologia - Compostos de Aroma
Enologia - Leveduras
Enologia - Técnicas de biologia molecular aplicada a microorganismos


EQUIPA

Agustín Aranda Fernández
Amparo Querol Simón
Eladio Barrio Esparducer
Emilia Matallana Redondo
Marcellí del Olmo Muñoz
Maria José Peris Torán
Maria Teresa Fernández Espinar García


PUBLICAÇÕES REPRESENTATIVAS

DE LLANOS, R,. FERNÁNDEZ-ESPINAR, M.T., QUEROL, A. (2004): Identification of species of the genus Candida by RFLP analysis of the 5.8S rRNA gene and the two ribosomal internal transcribed spacers. Antonie van Leeuwenhoek. 85:175-185.

DE LLANOS, R.; FERNÁNDEZ-ESPINAR, M.T.; PLANES, A.N.; QUEROL, A. (2004): Molecular characterization of clinical and non-clinical isolates of Saccharomyces cerevisiae. Systematic Appl. Microbiol. (En prensa)

ESTEVE-ZARZOSO, B., FERNÁNDEZ-ESPINAR, M.T., QUEROL, A.(2004): Authentication and identification of Saccharomyces cerevisiae "flor" yeast races involved in sherry aging. Antonie van Leeuwenhoek. 85:151-158.

MORRISSEY, W.F., QUEROL, A.; DOBSON, A. (2004): The role of indigenous yeasts in traditional Irish cider fermentations. J Appl Microbiol (Aceptado).

QUEROL, A., FERNÁNDEZ-ESPINAR, M.T.; BARRIO, E. (2004): Genetic variability of yeast in wine fermentation. In: Mycology, Vol. 21: Fungal Biotechnology in Agricultural, Food, an Environmental Applications. Marcer Dekker, Inc. (Dilip D. Arora, Ed.). Nerw York (USA). ISBN: 0-8247-4770-4. 257-267.

ZUZUARREGUI, A; DEL OLMO, M. (2004): Analyses of stress resistance under laboratory conditions constitute a suitable criterion for wine yeasts selection. Antonie van Leeuwnhoek. 85(4):271-280.

ZUZUAREGUI, A; DEL OLMO, M. (2004): Expression of stress response genes in wine strains with different fermentative behavior. Fems Yeast Res. 4(7): 699-710.

ALLER-ARRANZ, E., RANDEZ-GIL, F., BARRIO, E., PRIETO, JA. (2003): A DNA region of Torulaspora delbrueckii containing the HIS3 gene sequence, gene order and evolution. Yeast. 20(16): 1359-1368.

ARANDA, A.; DEL OLMO, M. (2003): Response to acetaldehyde stress in the yeast Saccharomyces cerevisiae involves a strain dependent regulation of several ALD genes and is mediated by the general stress response pathway. Yeast 20:747-759.

CARRASCO, P., PÉREZ-ORTÍN, J.E., DEL OLMO, M. (2003): Arginase activity is a useful marker of nitrogen limitation during alcoholic fermentations. Systematic Appl. Microbiol. 26: 471-479.

DIAS, L., DIAS, S., SANCHO, T., STENDER, H., QUEROL, A., MALFEITO-FERREIRA, M.; LOUREIRO, V. (2003): Identification of yeasts isolated from wine-related environments and capable of producing 4-ethylphenol. Food Microbiology. 20:567-574.

ESTEVE-ZARZOSO, B., ZORMAN, T., BELLOCH, C., AND QUEROL, A. (2003): Molecular characterisation of the species of the genus Zygosacccharomyces. System. Appl. Microbiol. 26:404-411.

FERNÁNDEZ-ESPINAR, T, BARRIO, E., RAMÓN D.; QUEROL, A. (2003): Analysis of the genetic variability in the species of Saccharomyces sensu stricto complex. Yeast. 20, 1213-1226.

MARTÍNEZ-CULEBRAS, P.V., QUEROL, A., SUÁREZ-FERNÁNDEZ, M.B., GARCÍA, M.D., BARRIO, E. (2003): Phylogenetic relationships among Colletotrichum pathogens of strawberry and design of PCR primers for their identification. J. Phytopathol. 151(3):135-143.

QUEROL, A., FERNÁNDEZ-ESPINAR, M.T., DEL OLMO, M., BARRIO, E. (2003): Molecular evolution in yeast of biotechnological interest. Int. Microbiol. 6, 201-205.

QUEROL, A., FERNÁNDEZ-ESPINAR, M.T., DEL OLMO, M., BARRIO, E. (2003): Adaptive evolution of wine yeast. Int. J. Food. Microbiol. 86: 3-10.

SÁEZ, A.G., TATARENKOV, A., BARRIO, E., BECERRA, N.H., AYALA, F.J. (2003): Patterns of DNA sequence polymorphism at Sod vicinities in Drosophila melanogaster: Unraveling the footprint of a recent selective sweep. Proc. Natl. Acad. Sci. USA. 100 (4): 1793-1798.

ALÉN, C., KENT, N.A., JONES, H.S., O'SULLIVAN, J., ARANDA, A. PROUDFOOT, N.J. (2002): A Role for Chromatin Remodeling in Transcriptional Termination by RNA Polymerase II. Mol. Cell. 10: 1441-1452.

ARANDA, A., QUEROL, A.; OLMO, M. DEL. (2002): Correlation between acetaldehyde and ethanol resistance and expression of HSP genes in yeast strains isolated during the biological aging of sherry wines. Arch Microbiol. 177: 304-312.

BELLOCH, C., FERNÁNDEZ-ESPINAR, T. QUEROL, A., GARCÍA, M.D. BARRIO, E. (2002): An analysis of inter-and intraspecific genetic variabilities in the K. marxianus group of yeast species for the reconsideration of the K. lactis taxon. Yeast. 19: 257-268.

CAMPBELL, S.G., DEL OLMO, M., BEGLAN, P., BOND, U. (2002): A sequence element downstream of the yeast HTB1 gene contributes to mRNA 3' processing and cell cycle regulation. Molecular and Cellular Biology. 22:8415-8425.

FARES, M.A., BARRIO, E., SABATER-MUÑOZ, B., MOYA, A. (2002): The evolution of the heat-shock protein GroEL from Buchnera, the primary endosymbiont of aphids, is governed by positive selection. Molecular Biology and Evolution 19:1162-1170.

FARES, M.A., ELENA, S.F., ORTIZ, J., MOYA, A., BARRIO, E. (2002): A sliding window-based method to detect selective constraints in protein coding genes: highlighting positively selected regions in RNA viruses. Journal of Molecular Evolution. 55:509-521.

FARES, M.A., RUIZ-GONZÁLEZ, M.X., MOYA, A., ELENA, S.F., BARRIO, E. (2002): Endosymbiotic bacteria: GroEL buffers against deleterious mutations. Nature 417:398.

GONZÁLEZ-ÁLVAREZ, M., ALZUET, G., BORRÁS, J., MACÍAS, B., DEL OLMO, M., LIU-GONZÁLEZ, M.; SANZ, F. (2002): Nuclease activity of (Cu(sulfathiazolato)2(benzimidazole)2)2MeOH. Synthesis, properties and crystal structure. Journal of Inorganic Biochemistry. 89: 29-35.

LOPES, C.A., BROOCK, M. VAN., QUEROL, A., CABALLERO, A.C. (2002): Saccharomyces cerevisiae wine yeast populations in a cold region in Argentinean Patagonia. A study at different fermentation scales. J Applied Microbiology. 93:608-615.

LÓPEZ, V., FERNÁNDEZ-ESPINAR, M.T., BARRIO, E., RAMÓN, D. QUEROL, A. (2002): A new PCR-based method for monitoring inoculated wine fermentations. Int J Food Microbiology. 81: 63-71.

MARTÍNEZ-CULEBRAS, P.V., SUÁREZ-FERNÁNDEZ, M.B., BARRIO, E., GARCÍA, M.D., QUEROL, A. (2002): RAPDs analysis of Colletotrichum species derived from strawberry and specific primers for identification of C. fragariae species. J. Phytopathol. 150:680-686.

PEDREÑO, Y., GIMENO-ALCAÑÍZ, J.V., MATALLANA, E., ARGÜELLES, J.C. (2002): Response to oxidative stress caused by H2O2 in saccharomyces cerevisiae mutants deficient in trehalase genes. Arch. Microbiol

PÉREZ-ORTÍN, J.E.; QUEROL A., PUIG, S.; BARRIO, E. (2002): Molecular Characterization of a Chromosomal Rearrangement Involved in the Adaptive Evolution of Wine Yeast Strains. Genome Research. 12:1533-1539.

PÉREZ-TORRADO, R., CARRACO, P., GIMENO-ALCAÑÍZ, J.V., PÉREZ-ORTÍN, J.E., MATALLANA, E.; DEL OLMO, M. (2002): Study of the first hours of microvinification by the use of osmotic stress-response genes as probes. System. Appl. Microbiol. 25:153-161.

PÉREZ-TORRADO, R., GIMENO-ALCAÑIZ, J.V. MATALLANA, E. (2002): Wine yeast strains engineered for glycogen overproduction display enhanced viability under glucose deprivation conditions. Appl. Environm. Microbiol.

ARANDA, A., PROUDFOOT, N.J. (2001): Transcriptional termination factors for RNA polynerase II in yeast. Mol. Cell. 7:1003-1011.

CARRASCO, P., QUEROL, A., DEL OLMO, M. (2001): Analysis of the stress resistance of commercial wine yeast strains. Arch Microbiol. 175, 450-457.

ESTEVE-ZARZOSO, B., PERIS-TORÁN, M.J., GARCÍA-MAIQUEZ, E., URUBURU, F. ; QUEROL, A. (2001): Yeast population dynamics during the fermentation and biological aging of sherry wines. Appl. Environ. Microbiol. 67(5), 2056-2061.

ESTEVE-ZARZOSO, B., PERIS-TORÁN, M.J., RAMÓN, D., QUEROL, A. (2001): Molecular characterization of Hanseniaspora species. Antonie van Leeuwenhoek. 80: 85-92.

FARES, M.A., MOYA, A., ESCARMÍS, C., BARANOWSKI, E., DOMINGO, E., BARRIO, E. (2001): Evidence for positive selection in the capsid protein-coding region of the foot-and-mouth disease virus (FMDV) subjected to experimental passage regimens. Molecular Biology and Evolution. 18(1): 10-21.

FERNÁNDEZ-ESPINAR, M.T., LÓPEZ, V., RAMÓN, D., BARTRA, E., QUEROL, A. (2001): Study of the authenticity of commercial wine yeast strains by molecular techniques. International Journal of Food Microbiology. 70:1-10.

GANGA, A., PIÑAGA, F., QUEROL, A., RAMÓN, D. VALLÉS, S. (2001): Cell-wall degrading enzymes in the release of grape aroma precursors. Food Sci. Technol. Int. 7(1):83-87.

GIMENO-ALCAÑIZ, J.V., MATALLANA, E. (2001): Performance of industrial strains of Saccharomyces cerevisiae during wine fermentation is affected by manipulation strategies based on sporulation. System. Appl. Microbiol. 24, 639-644.

LÓPEZ, V., QUEROL, A., RAMÓN, D., FERNÁNDEZ-ESPINAR, M.T. (2001): A simplified procedure to analyse mitochondrial DNA from industrial yeasts. International Journal of Food Microbiology. 68:75-81.

MARÍN, I., FARES, M.A., GONZÁLEZ-CANDELAS, L., BARRIO, E., MOYA, A. (2001): Detecting changes in the functional constrints of paralogous genes. Journal of Molecular Evolution. 52:17-28.

BELLOCH, C., QUEROL, A., GARCÍA, M.D., BARRIO, E. (2000): Phylogeny of the genus Kluyveromyces inferred from the mitochondrial cytochrome-c oxidase II gene. Int. J. of Syst. and Evolutionary Microbiology. 50: 405-416.

ESTEVE-ZARZOSO, B.; GOSTÍNCAR, A.; BOBET, R.; URUBURU, F.; QUEROL, A. (2000): Selection and molecular characterization of wine yeasts isolated from "El Penedes" area (Spain). Food Microbiology. 17:553-562.

FERNÁNDEZ-ESPINAR, M.T., ESTEVE-ZARZOSO, B., QUEROL, A. BARRIO, E. (2000): RFLP analysis of the ribosomal internal transcribed spacer and the 5.8S rRNA gene region of the genus Saccharomyces: a fast method for species identification and the differentiation of flor yeasts. Anton Leeuw Int. J.G. 78:87-97.

FERNÁNDEZ-ESPINAR, T., QUEROL, A., RAMÓN, D. (2000): Molecular characterization of yeast strains by mitochondrial DNA restriction analysis. ISBN 0-89603-867-X. Methods in Biotechnology.

GREGER, I.H., ARANDA, A.; PROUDFOOT, N.J. (2000): Balancing transcriptional interference and initiation on the GAL7 promoter of Saccharomyces cerevisiae. Proc.Natl. Acad. Sci. USA. 97: 8415-8420.

MARTÍNEZ-CULEBRAS, P.V., BARRIO, E., GARCÍA-LÒPEZ, M.D., QUEROL, A. (2000): Identification of Collectotrichum species responsible for anthracnose of strawberry based on the internal transcribed spacers of the ribosomal region. FEMS Microbiology Letters. 189: 97-101.

MOYA, A., ELENA, S.F., BRACHO, A., MIRALLES, R., BARRIO, E. (2000): The evolution of RNA viruses: a population genetics view. Proc. Natl. Acad. Sci. USA 97(13):6967-6973.

PERAPOCH, J., PLANES, A.M., QUEROL, A., LÓPEZ, V., MARTÍNEZ-BENDAYÁN, I., TORMO, R., FERNÁNDEZ, F., PEGUERO, G. SALCEDO, S. (2000): Fungemia with Saccharomyces cerevisiae in two newborns, only one of whom had been treated with ultra-levura. Eur J Clin Microbiol Infect Dis. 19: 468-470.

PUIG, S., QUEROL, A., BARRIO, E., PÉREZ-ORTÍN, J.E. (2000): Mitotic recombination and genetic changes in Saccharomyces cerevisiae during wine fermentation. Applied and Environmental Microbiology. 66(5): 2057-2061.

QUEROL, A. (2000): Modificació genètica de llevats vínics. ACE Revista d'Enologia. 52: 16-19.

QUEROL, A., ESTEVE-ZARZOSO, B., LÓPEZ, V., FERNÁNDEZ-ESPINAR, M.T., BARRIO, E., PERIS-TORÁN, M.J.; RAMÓN, D. (2000): Molecular methods for the characterisation and monitoring of wine yeasts. Inoculation Rate and nutritional aspects keys points for food alcoholic fermentation management. (Symposium LALLEMAND). 35-41.

VAN HELDEN, J., DEL OLMO, M., PÉREZ-ORTÍN, J.E. (2000): Statistical analysis of yeast genomic downstream sequences reveals putative polyadenylation signals. Nuc. Acids Res. 28: 1000-1010.

ARANDA, A., PROUDFOOT, N.J. (1999): Definition of transcriptional pause elements in fission yeast. Mol. Cell. Biol. 19: 1595-1604.

BUDDIE, A. G., MARTÍNEZ-CULEBRAS, P., BRIDGE, P. D., GARCÍA, M. D., QUEROL, A., CANNON, P. F., MONTE, E. (1999): Molecular characterization of Colletotrichum strains derived from strawberry. Mycol. Res. 103:385-394.

ESTEVE-ZARZOSO, B., BELLOCH, C., URUBURU, F. , QUEROL, A. (1999): Identification of yeast by RFLP analysis of the 5.8S rRNA gene and the two ribosomal internal transcribed spacers. Int. J. Syst. Bacteriol. 49:329-337.

GANGA, M.A., PIÑAGA, F., VALLÉS, S., RAMÓN, D., QUEROL, A. (1999): Aroma improving in microvinification processes by the use of a recombinant wine yeast strain expressing the Aspergillus nidulans xlnA gene. International Journal of Food Microbiology. 47:171-178.

GASENT-TAMÍREZ, CASTREEJÓN, F., QUEROL, A., RAMÓN, D., BENÍTEZ, T. (1999): Genomic stability of Saccharomyces cerevisiae baker´s yeasts. System. Appl. Microbiol. 22: 329-340.

GIMENO-ALCAÑIZ, J.V.; PÉREZ-ORTÍN, J.E., MATALLANA, E. (1999): Differential pattern of trehalose accumulation in wine yeast strains during the microvinification process. Biotechnol. Lett. 21: 271-274.

IVORRA, C., PÉREZ-ORTÍN, J.E., DEL OLMO, M. (1999): Reverse correlation of stress-resistance ability and stuck fermentations in wine yeasts. A molecular study. Biotechnol. & Bioeng. 64: 678-708.

LOUREIRO, V., QUEROL, A. (1999): The prevalence and control of spoilage yeasts in foods and beverages. Trends in Food Science and Technology. 10, 356-365.

MANZANARES, P., RAMÓN, D., QUEROL, A. (1999): Screening of non-Saccharomyces wine yeasts for the production of b-D-xylosidase activity. International. J. Food Microbiol. 46, 105-112.

PUIG, S.; MATALLANA, E., PÉREZ-ORTÍN, J.E. (1999): Stochastical mucleosome positioning in a yeast chromayin region is not dependent on histone H1. Curr. Microbiol. 39:168-172.

PUIG, S.; PÉREZ-ORTÍN, J.E., MATALLANA, E. (1999): Transcriptional and structural study of a region of two convergent overlapping yeast genes. Curr. Microbiol. 39:369-373.

TATARENKOV, A., KWIATOWSKI, J., SKARECKY, D., BARRIO, E., AYALA, F.J. (1999): On the evolution of Ddc and Drosophila Systematics. Journal of Molecular Evolution. 48:445-462.

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